Table 1 : monoclonal antibodies developed toward murine p53
Table 2: monoclonal antibodies developped toward other p53 that cross react with murine p53

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

 (1) Recognition based on peptide binding. Cross reaction of 200.27 to human p53 has been the subject of debate (see references )
(2) PAb246, like PAb1620, binds a conformational epitopes wich disapears upon denaturation or in some mutant p53

see references for more information

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

References

• 1 Gurney EG, Harrison RO and Fenno J (1980) Monoclonal antibodies against simian virus 40 T antigens : evidence for distinct subclasses of large T antigen and for similaraties among nonviral T antigens. J. Virol. 34 : 752-763.
• 2 Harlow E, Crawford LV, Pim DC and Williamson NM (1981) Monoclonal antibodies specific for Simian Virus 40 tumor antigens. J. Virol. 861-869.
• 4 Wade-Evans A and Jenkins JR (1985) Precise epitope mapping of the murine transformation-associated protein, p53. EMBO J. 4 : 699-706.
• 6 Yewdell JW, Gannon JV and Lane DP (1986) Monoclonal antibody analysis of p53 expression in normal and transformed cells. J. Virol. 59 : 444-452.
• 7 Gannon JV, Greaves R, Iggo R and Lane DP (1990) Activating mutations in p53 produce a common conformational effect - A monoclonal antibody specific for the mutant form. EMBO J. 9 : 1595-1602.
• 8 Stephen CW and Lane DP (1992) Mutant conformation of p53 - precise epitope mapping using a filamentous phage epitope library. J Mol Biol 225 : 577-583.
• 11 Legros Y, Lacabanne V, D'Agay MF, Larsen CJ, Pla M and Soussi T (1993) Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells. Bull. du Cancer 80 : 102-110.
• 12 Legros Y, Lafon C and Soussi T (1994) Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Oncogene 9 : 2071-2076.
• 13 Legros Y, Meyer A, Ory K and Soussi T (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694.
• 15 Stephen, C. W., Helminen, P., and Lane, D. P. (1995). Characterisation of epitopes on human p53 using phage-displayed peptide libraries : insights into antibody peptide interactions. J Mol Biol 248, 58-78.
• 16 Vojtesek B, Dolezalova H, Lauerova L, Svitakova M, Havlis P, Kovarik J, Midgley CA and Lane DP (1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein. Oncogene 10 : 389-393.
• 17 Lane DP, Stephen CW, Midgley CA, Sparks A, Hupp TR, Daniels DA, Greaves R, Reid A, Vojtesek B and Picksley SM (1996) Epitope analysis of the murine p53 tumour suppressor protein. Oncogene 12 : 2461-2466.
• 18 Dolezalova H, Vojtesek B and Kovarik J (1997) Epitope analysis of the human p53 tumour suppressor protein. Folia Biol Prague 43 : 49-51.
• 19 Legros and Soussi, Unpublished results
• 20 Coffman RL and Weissman IL (1981) A monoclonal antibody that recognizes B cells and B cell precursors in mice. J Exp Med 153 : 269-279.
• 21 Dippold WG, Jay G, DeLeo AB, Khoury G and Old LJ (1981) p53 transformation-related protein : detection by monoclonal antibody in mouse and human cells. Proc. Natl. Acad. Sci. USA 78 : 1695-1699.