p53 Information

p53 information

p53 story

p53 monoclonal antibodies

Human

Monkey

Mouse

Rabbit

Hasmter

Rat

Chicken

Xenopus

Other

 

 

 

 

 

 

 

 

 

p53 pathways

p53 gene

p53 protein(s)

p63/p73 protein(s)

p53 evolution

p53 polymorphism

p53 and cancer

mouse models

Rat p53

No monoclonal have been developped toward rat p53.
Most of the monoclonal antibodies that binds to murine p53 binds also to rat p53.

There are a few exceptions with monoclonal antibodies which binds to rat p53 but does not recognize murine p53 (table 2)

Table 1a and 1b : rat and mouse p53 monoclonal antibodies

Table 2 : monoclonal antibodies which binds rat p53 but no mouse p53

 

Table 1a : Rat and mouse p53 monoclonal antibodies
(mouse p53 used for immunization)

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Reference

Comercial

availability

PAb242

18-27

Mouse p53

IgG1

No

Yes

Yes

6, 16

PAb248

43-52

Mouse p53

IgG2b

No

Yes

Yes

4, 6, 16

200.47

78-87

Mouse p53

IgG2a

weak human (1)

Yes

Yes

20, 16

No

PAb240

213-217

Mouse p53

IgG1

Yes

Yes

Yes

6, 7, 8

Yes

PAb122

371-380

Mouse p53

IgG2b

Yes

Yes

Yes

1,4,15

Yes

PAb421

371-380

Mouse p53

IgG2a

Yes

Yes

Yes

2,4,15

Yes

PAb246

?(2)

Mouse p53

IgG1

No

No

Yes

6, 7, 16

Yes

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

(1) Recognition based on peptide binding. Cross reaction of 200.27 to human p53 has been the subject of debate (see references )
(2) PAb246, like PAb1620, binds a conformational epitopes wich disapears upon denaturation or in some mutant p53

see references for more information

Table 1b : Rat and mouse p53 monoclonal antibodies
(human p53 used for immunization)

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Reference

Comercial

availability

B17

16-25

Human p53

IgG2a

Yes

Yes

Yes

11, 12

No

X77

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

18

No

X73

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

18

No

X18

16-25

Xenopus p53

IgG1

Yes

Yes

Yes

18

No

HP64

171-185

Human p53

IgG1

Yes

Yes

Yes

11, 12

No

HO13.1

211-220

Human p53

IgG2b

Yes

Yes

Yes

13

No

HO8.1

306-361

Human p53

IgG1

Yes

Yes

Yes

13

Yes

HR231

371-380

Human p53

IgG2b

Yes

Yes

Yes

11, 12

No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

Table 2 : Mabs that distinguish rat from mouse p53

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Reference

Comercial

availability

C36

16-30

Human p53

IgG1

Yes

Yes

Yes

11, 12

No

H461

16-30

Human p53

IgG1

Yes

Yes

Yes

11, 12

No

X87

16-30

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

X44

16-30

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

X61

16-30

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

X91

16-30

Xenopus p53

IgG1

Yes

Yes

Yes

19

No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

References

  • 1 Gurney EG, Harrison RO and Fenno J (1980) Monoclonal antibodies against simian virus 40 T antigens : evidence for distinct subclasses of large T antigen and for similaraties among nonviral T antigens. J. Virol. 34 : 752-763.
  • 2 Harlow E, Crawford LV, Pim DC and Williamson NM (1981) Monoclonal antibodies specific for Simian Virus 40 tumor antigens. J. Virol. 861-869.
  • 4 Wade-Evans A and Jenkins JR (1985) Precise epitope mapping of the murine transformation-associated protein, p53. EMBO J. 4 : 699-706.
  • 6 Yewdell JW, Gannon JV and Lane DP (1986) Monoclonal antibody analysis of p53 expression in normal and transformed cells. J. Virol. 59 : 444-452.
  • 7 Gannon JV, Greaves R, Iggo R and Lane DP (1990) Activating mutations in p53 produce a common conformational effect - A monoclonal antibody specific for the mutant form. EMBO J. 9 : 1595-1602.
  • 8 Stephen CW and Lane DP (1992) Mutant conformation of p53 - precise epitope mapping using a filamentous phage epitope library. J Mol Biol 225 : 577-583.
  • 11 Legros Y, Lacabanne V, D'Agay MF, Larsen CJ, Pla M and Soussi T (1993) Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells. Bull. du Cancer 80 : 102-110.
  • 12 Legros Y, Lafon C and Soussi T (1994) Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Oncogene 9 : 2071-2076.
  • 13 Legros Y, Meyer A, Ory K and Soussi T (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694.
  • 15 Stephen, C. W., Helminen, P., and Lane, D. P. (1995). Characterisation of epitopes on human p53 using phage-displayed peptide libraries : insights into antibody peptide interactions. J Mol Biol 248, 58-78.
  • 17 Lane DP, Stephen CW, Midgley CA, Sparks A, Hupp TR, Daniels DA, Greaves R, Reid A, Vojtesek B and Picksley SM (1996) Epitope analysis of the murine p53 tumour suppressor protein. Oncogene 12 : 2461-2466.
  • 19 Legros and Soussi, Unpublished results
 
 
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