p53 Information

p53 information

p53 story

p53 monoclonal antibodies

Human

Monkey

Mouse

Rabitt

Hasmter

Rat

Chicken

Xenopus

Other

 

 

 

 

 

 

 

 

 

p53 pathways

p53 gene

p53 protein(s)

p63/p73 protein(s)

p53 evolution

p53 polymorphism

p53 and cancer

mouse models

Chicken p53

In our hands, only one monoclonal antibody reacts with Chicken p53.

Name

Epitope Location

Antigen used

for immunization

Isotype

Other species

recognition

WB

IP

Comercial

availability

HP64

171-185

Human p53

IgG1

Yes

Yes

Yes

No

p53 analysis by western bloting using HP64 monoclonal antibody

p53_antibodies

lane 1: Saos-2 cells : Osteosarcoma cell line with no endogenous p53
lane 2: SW480 cells; Colorectal carcinoma cell line with mutant p53
lane 3: Tcf1 cells; Chicken cells transformed by SV40
lane 4: COS cells; monkey cells transformed by SV40
lane 5: SVT2; mouse cells transformed by SV40

 

References

  • 12 Legros Y, Lafon C and Soussi T (1994) Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Oncogene 9 : 2071-2076.
  • 13 Legros Y, Meyer A, Ory K and Soussi T (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694.
  • 19 Legros and Soussi, Unpublished results

     

 
 
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