In our hands, only one monoclonal antibody reacts with Chicken p53.
p53 analysis by western bloting using HP64 monoclonal antibody
lane 1: Saos-2 cells : Osteosarcoma cell line with no endogenous p53 lane 2: SW480 cells; Colorectal carcinoma cell line with mutant p53 lane 3: Tcf1 cells; Chicken cells transformed by SV40 lane 4: COS cells; monkey cells transformed by SV40 lane 5: SVT2; mouse cells transformed by SV40
12 Legros Y, Lafon C and Soussi T (1994) Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Oncogene 9 : 2071-2076.
13 Legros Y, Meyer A, Ory K and Soussi T (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694.