p53 Information

p53 information

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p53 monoclonal antibodies

Human

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p53 pathways

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p53 protein(s)

p63/p73 protein(s)

p53 evolution

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p53 and cancer

mouse models

HUMAN p53

Due to the large number of monoclonal antibodies which recognized human p53, the table has been seperated in three parts :

Human p53 - amino-terminus

Name Epitope Location Antigen used for immunization Isotype Other species recognition WB IP Reference Comercial availability
Bp53-1 6-15 Human p53 IgG1 ? Yes Yes 10, 18 ?
Bp53-31 6-15 Human p53 IgG3 ? Yes Yes 10, 18 ?
Bp53-8 6-15 Human p53 IgG1 ? Yes Yes 10, 18 ?
Bp53-22 6-20 Human p53 IgG1 ? Yes Yes 10, 18 ?
Bp53-25 6-20 Human p53 IgG1 ? Yes Yes 10, 18 ?
Bp53-27 6-20 Human p53 IgG1 ? Yes Yes 10, 18 ?
DO2 10-16 Human p53 IgG ? Yes Yes 9, 15 Yes
L134 11-20 Human p53 IgG1 No Yes Yes 11, 12 No
B17 16-25 Human p53 IgG2a Yes Yes Yes 11, 12 No
X77 16-25 Xenopus p53 IgG1 Yes Yes Yes 19 No
X73 16-25 Xenopus p53 IgG1 Yes Yes Yes 19 No
X18 16-25 Xenopus p53 IgG1 Yes Yes Yes 19 No
Bp53-2 16-25 Human p53 IgG1 ? Yes Yes 10, 18 No
Bp53-2 16-25 Human p53 IgG1 ? Yes Yes 10, 18 No
Bp53-13 16-25 Human p53 IgG2a ? Yes Yes 10, 18 No
Bp53-24 16-25 Human p53 IgG2b ? Yes Yes 10, 18 No
Bp53-28 16-25 Human p53 IgG2a ? Yes Yes 10, 18 No
C36 16-30 Human p53 IgG1 Yes Yes Yes 11, 12 No
H461 16-30 Human p53 IgG1 Yes Yes Yes 11, 12 No
X87 16-30 Xenopus p53 IgG1 Yes Yes Yes 18 No
X44 16-30 Xenopus p53 IgG1 Yes Yes Yes 18 No
X61 16-30 Xenopus p53 IgG1 Yes Yes Yes 18 No
X91 16-30 Xenopus p53 IgG1 Yes Yes Yes 18 No
Bp53-14 16-30 Human p53 IgG2a ? Yes Yes 10, 18 No
DO7 21-25 Human p53 IgG2b ? Yes Yes 9, 15 Yes
DO1 21-25 Human p53 IgG2a ? Yes Yes 9, 15 Yes
Bp53-4 21-25 Human p53 IgG1 ? Yes Yes 10, 18 No
BP53-12 20-25 Human p53 IgG2a ? Yes Yes 10, 18 Yes
BP53-11 20-25 Human p53 IgG2a ? Yes Yes 10, 18 No
BP53-19 20-23 Human p53 IgG Yes Yes Yes 10, 18 No
DO13 26-35 Human p53 IgG1 ? Yes Yes 9, 16 No
PAb1801 46-55 Human p53 IgG1 No Yes Yes 3, 5, 12 Yes
H447 46-55 Human p53 IgG1 No Yes Yes 11, 12 No
H279 46-55 Human p53 IgG1 No Yes Yes 11, 12 No
DO14 56-65 Human p53 IgG1 ? Yes Yes 16 No
Bp53-20 56-65 Human p53 IgG1 ? Yes Yes 10, 18 No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

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Human p53 - central region

Name Epitope
Location		 Antigen use for immunization Isotype Other species recognition WB IP Reference Comercial availability
HO15.4 86-95 Human p53 IgG2a No Yes Yes 13 No
HO16.4 86-95 Human p53 IgG2a No Yes Yes 13 No
HP64 171-185 Human p53 IgG1 Yes Yes Yes 11,12 No
HO3.5 181-190 Human p53 IgG1 No Yes Yes 13 No
HO31.1 181-190 Human p53 IgG1 No Yes Yes 13 No
DO11 181-190 Human p53 IgG1 ? Yes Yes 16 No
HO13.1 211-220 Human p53 IgG2b Yes Yes Yes 12 No
PAb240 213-217 Murine p53 IgG1 Yes Yes Yes 6,7,8 Yes
DO12 256-270 Human p53 IgG1 ? Yes Yes 16 No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

TOP OF THE PAGE

Human p53 - carboxy-terminus

Name Epitope Location Antigen used for immunization Isotype Other species recognition WB IP Reference Comercial availability
HO7.1 291-300 Human p53 IgG2a No Yes Yes 13 No
HO33.8 291-300 Human p53 IgG2a No Yes Yes 13 No
HO8.1 306-361 Human p53 IgG1 Yes Yes Yes 13 Yes
PAb122 371-380 Mouse p53 IgG2b Yes Yes Yes 1, 4, 15 Yes
PAb421 371-380 Mouse p53 IgG2a Yes Yes Yes 2, 4, 15 Yes
HR231 371-380 Human p53 IgG2b Yes Yes Yes 10, 11 No
Bp53-3 371-380 Human p53 IgG2a ? Yes Yes 10, 18 No
Bp53-30 371-380 Human p53 IgG2b ? Yes Yes 10, 18 No
Bp53-6 376-380 Human p53 IgG1 ? Yes Yes 10, 18 No
ICA9 388-393 synthetic peptide IgG ? ? Yes 14 No

Only mAbs that have been experimentally tested either by ELISA, immunoprecipitation or western blot are listed on these tables.
From the epitope sequence, it is quite possible that other mAbs react with this species.

References

  • 1 Gurney EG, Harrison RO and Fenno J (1980) Monoclonal antibodies against simian virus 40 T antigens : evidence for distinct subclasses of large T antigen and for similaraties among nonviral T antigens. J. Virol. 34 : 752-763.
  • 2 Harlow E, Crawford LV, Pim DC and Williamson NM (1981) Monoclonal antibodies specific for Simian Virus 40 tumor antigens. J. Virol. 861-869.
  • 3 Leppard K and Crawford L (1983) Monoclonal antibodies displaying a novel species specificty for the primate transformation-related protein p53. EMBO J. 2 : 1457-1464.
  • 4 Wade-Evans A and Jenkins JR (1985) Precise epitope mapping of the murine transformation-associated protein, p53. EMBO J. 4 : 699-706.
  • 5 Banks L, Matlashewski G and Crawford L (1986) Isolation of human-p53 specific monoclonal antibodies and their use in the studies of human p53 expression. Eur. J. Biochem. 159 : 529-534.
  • 6 Yewdell JW, Gannon JV and Lane DP (1986) Monoclonal antibody analysis of p53 expression in normal and transformed cells. J. Virol. 59 : 444-452.
  • 7 Gannon JV, Greaves R, Iggo R and Lane DP (1990) Activating mutations in p53 produce a common conformational effect - A monoclonal antibody specific for the mutant form. EMBO J. 9 : 1595-1602.
  • 8 Stephen CW and Lane DP (1992) Mutant conformation of p53 - precise epitope mapping using a filamentous phage epitope library. J Mol Biol 225 : 577-583.
  • 9 Vojtesek B, Bartek J, Midgley CA and Lane DP (1992) An Immunochemical Analysis of the Human Nuclear Phosphoprotein-p53 - New Monoclonal Antibodies and Epitope Mapping Using Recombinant-p53. J Immunol Methods 151 : 237-244.
  • 10 Bartek J, Bartkova J, Lukas J, Staskova Z, Vojtesek B and Lane DP (1993) Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies. J Pathol 169 : 27-34.
  • 11 Legros Y, Lacabanne V, D'Agay MF, Larsen CJ, Pla M and Soussi T (1993) Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells. Bull. du Cancer 80 : 102-110.
  • 12 Legros Y, Lafon C and Soussi T (1994) Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein. Oncogene 9 : 2071-2076.
  • 13 Legros Y, Meyer A, Ory K and Soussi T (1994) Mutations in p53 produce a common conformational effect that can be detected with a panel of monoclonal antibodies directed toward the central part of the p53 protein. Oncogene 9 : 3689-3694.
  • 14 Hupp TR and Lane DP (1995) Two distinct signaling pathways activate the latent DNA binding function of p53 in a casein kinase II-independent manner. J Biol Chem 270 : 18165-18174.
  • 15 Stephen, C. W., Helminen, P., and Lane, D. P. (1995). Characterisation of epitopes on human p53 using phage-displayed peptide libraries : insights into antibody peptide interactions. J Mol Biol 248, 58-78.
  • 16 Vojtesek B, Dolezalova H, Lauerova L, Svitakova M, Havlis P, Kovarik J, Midgley CA and Lane DP (1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein. Oncogene 10 : 389-393.
  • 17 Lane DP, Stephen CW, Midgley CA, Sparks A, Hupp TR, Daniels DA, Greaves R, Reid A, Vojtesek B and Picksley SM (1996) Epitope analysis of the murine p53 tumour suppressor protein. Oncogene 12 : 2461-2466.
  • 18 Dolezalova H, Vojtesek B and Kovarik J (1997) Epitope analysis of the human p53 tumour suppressor protein. Folia Biol Prague 43 : 49-51.
  • 19 Legros and Soussi, Unpublished results
 
 
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