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Cell lines database
p53 database
p53 mutations in cell lines
The hanbook of p53 mutation in cell lines
Cell lines with a controversial p53 status
The NCI 60 panel
Bladder cancer cell lines
Brain cancer cell lines
Breast cancer cell lines
Cervical carcinoma
Colorectal cancer cell lines
Esophageal cancer cell lines
Gastric cancer cell lines
Head and Neck cancer cell lines
Leukemia / Lymphoma cell lines
NSCLC cancer cell lines
Ovarian cancer cell lines
Pancreatic cancer cell lines
Sarcoma cell lines
SCLC cancer cell lines
Melanoma cell lines
Hepatocellular carcinoma
References
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p53 mutations in cell lines: main page
The problem
Cancer cell lines are essential tools used in many areas of biomedical research. Using the last release of the UMD_p53 database, we analysed the p53 status of 1,211 cell lines published between 1989 and 2007. . Strikingly, we found discrepancies in the p53 status for 23% (88/384) of cell lines, for which the p53 status was established independently in two laboratories. Using the NCI-60 cell line panel as a model widely used in the literature, the p53 status could not be ascertained for 13 cell lines due to a lack of homogeneous data in the literature. Our study clearly confirms that misidentified cell lines are still a silent and neglected danger and that extreme care should be taken as a wrong p53 status could lead to disastrous experimental interpretations. The p53 web site has been updated with new sections describing the p53 status in the majority of cell lines and a special section devoted to cell lines with controversial p53 status.
Berglind H, Pawitan Y, Kato S, Ishioka C, Soussi T (2008) Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination. Cancer Biol Ther, 7: 699–708
download the manuscript
The Database
p53 mutations in cell lines |
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Finding information
As shown in the left panel, you can find information for each type of cancer. All data are included in the handbook of p53 mutations in human cancer cell lines that can be downloaded here. Cell lines with a controversial p53 status have been highlighted.
Some recommendations
- Avoid using cell lines with an "uncertain origin"
- Always check the p53 status of a novel cell line
- Check the p53 status of your cell lines. RT-PCR and DNA sequencing is now rapid, easy and inexpensive.
- Sequence the entire p53 gene. In the case of unusual mutations (“neutral mutations”, mutations close to the splice junction), check the RNA.
- Observe strict rules for cell culture maintenance to avoid cross-contamination.
Improving the situation
You are strongly encouraged to share your data with us for the benefit of the scientific community.
All suggestions or input to improve the cell linse database are also welcome: p53@free.fr
You can find more information about Cross Contamination at the WIKI site that lists every contaminated cell lines ->Click
The Call of Action by Roland Nardone for the eradication of Cross Contaminated cell lines -> Click
the COSMIC database -> Click
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