Cell lines database

p53 database

p53 mutations in cell lines

The hanbook of p53 mutation in cell lines

Cell lines with a controversial p53 status

The NCI 60 panel

Bladder cancer cell lines

Brain cancer cell lines

Breast cancer cell lines

Cervical carcinoma

Colorectal cancer cell lines

Esophageal cancer cell lines

Gastric cancer cell lines

Head and Neck cancer cell lines

Leukemia / Lymphoma cell lines

NSCLC cancer cell lines

Ovarian cancer cell lines

Pancreatic cancer cell lines

Sarcoma cell lines

SCLC cancer cell lines

Melanoma cell lines

Hepatocellular carcinoma

References

 

 p53 mutations in cell lines: main page

The problem

Cancer cell lines are essential tools used in many areas of biomedical research. Using the last release of the UMD_p53 database, we analysed the p53 status of 1,211 cell lines published between 1989 and 2007. . Strikingly, we found discrepancies in the p53 status for 23% (88/384) of cell lines, for which the p53 status was established independently in two laboratories. Using the NCI-60 cell line panel as a model widely used in the literature, the p53 status could not be ascertained for 13 cell lines due to a lack of homogeneous data in the literature. Our study clearly confirms that misidentified cell lines are still a silent and neglected danger and that extreme care should be taken as a wrong p53 status could lead to disastrous experimental interpretations. The p53 web site has been updated with new sections describing the p53 status in the majority of cell lines and a special section devoted to cell lines with controversial p53 status.

Berglind H, Pawitan Y, Kato S, Ishioka C, Soussi T (2008) Analysis of p53 mutation status in human cancer cell lines: a paradigm for cell line cross-contamination. Cancer Biol Ther, 7: 699–708
download the manuscript

The Database

p53 mutations in cell lines

Finding information

As shown in the left panel, you can find information for each type of cancer. All data are included in the handbook of p53 mutations in human cancer cell lines that can be downloaded here. Cell lines with a controversial p53 status have been highlighted.

Some recommendations

  • Avoid using cell lines with an "uncertain origin"
  • Always check the p53 status of a novel cell line
  • Check the p53 status of your cell lines. RT-PCR and DNA sequencing is now rapid, easy and inexpensive.
  • Sequence the entire p53 gene. In the case of unusual mutations (“neutral mutations”, mutations close to the splice junction), check the RNA.
  • Observe strict rules for cell culture maintenance to avoid cross-contamination.

Improving the situation

You are strongly encouraged to share your data with us for the benefit of the scientific community.
All suggestions or input to improve the cell linse database are also welcome: p53@free.fr

You can find more information about Cross Contamination at the WIKI site that lists every contaminated cell lines ->Click

The Call of Action by Roland Nardone for the eradication of Cross Contaminated cell lines -> Click

the COSMIC database -> Click

 
 
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